|本期目录/Table of Contents|

[1]田坤云,崔学锋,张瑞林.低氧条件下好氧型微生物降解煤吸附甲烷实验[J].中国安全生产科学技术,2016,12(4):30-34.[doi:10.11731/j.issn.1673-193x.2016.04.006]
 TIAN Kunyun,CUI Xuefeng,ZHANG Ruilin.Experiment on degradation of methane adsorption in coal by aerobic microorganism under low oxygen condition[J].JOURNAL OF SAFETY SCIENCE AND TECHNOLOGY,2016,12(4):30-34.[doi:10.11731/j.issn.1673-193x.2016.04.006]
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低氧条件下好氧型微生物降解煤吸附甲烷实验
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《中国安全生产科学技术》[ISSN:1673-193X/CN:11-5335/TB]

卷:
12
期数:
2016年4期
页码:
30-34
栏目:
学术论著
出版日期:
2016-04-30

文章信息/Info

Title:
Experiment on degradation of methane adsorption in coal by aerobic microorganism under low oxygen condition
作者:
田坤云1崔学锋2张瑞林1
1.河南工程学院 安全工程学院,河南 郑州 451191;
2.河南理工大学 安全科学与工程学院,河南 焦作 454003)
Author(s):
TIAN Kunyun1 CUI Xuefeng2 ZHANG Ruilin1
(1. College of Safety Engineering, Henan Institute of Engineering, Zhengzhou Henan 451191, China;
2. College of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo Henan 454003, China)
关键词:
甲烷氧化菌好氧型低氧环境降解效能
Keywords:
methane oxidizing bacteria aerobic type low oxygen environment degradation efficiency
分类号:
X936
DOI:
10.11731/j.issn.1673-193x.2016.04.006
文献标志码:
A
摘要:
为了研究好氧型微生物对低氧气浓度环境条件下煤吸附甲烷的降解效能,培养、分离、初步鉴定了高效降解甲烷的好氧型甲烷氧化菌。并在高压容量法瓦斯吸附-解吸装置的基础上,自主开发了低氧环境下甲烷降解实验分析系统,研究了氧气浓度为0%、5%和15%三种条件下甲烷氧化菌的降解效能,通过对实验前后甲烷减少量,二氧化碳增加量,氧气减少量进行分析。结果表明:在氧气浓度(0%~15%)范围内,随着氧气浓度升高及降解时间的持续,甲烷的减少量可达130.5 cm3,二氧化碳的增加量最高可达25.7 cm3,同时最多消耗69.0 cm3氧气;在无氧条件下,好氧型甲烷氧化菌的生理活性受到了一定的限制,但最高仍然可以降解11.9 cm3的甲烷,生成二氧化碳3.5 cm3。
Abstract:
In order to study the degradation efficiency of aerobic microorganism on methane adsorption in coal under low oxygen environment condition, the aerobic methane oxidizing bacteria for degradation of methane with high efficiency was cultivated, isolated and preliminary identified. An experimental analysis system on methane degradation under low oxygen environment was self-developed based on the methane adsorption and desorption device using high-pressure volumetric method, and the degradation efficiencies of methane oxidizing bacteria under the conditions of 0%, 5% and 15% oxygen concentration were studied. By comparing the decrement of methane, increment of carbon dioxide and decrement of oxygen before and after experiment, it showed that in the range of oxygen concentration (0~15%), with the increase of oxygen concentration and the duration of degradation time, the decrement of methane could reach 130.5 cm3, the maximum increment of carbon dioxide could reach 25.7 cm3, while 69 cm3 oxygen could be consumed at most. Under the condition of anaerobic environment, the physiological activity of aerobic methane oxidizing bacteria was subject to certain restrictions, but the maximum degradation amount of methane could still reach 11.9 cm3, and 3.5 cm3 carbon dioxide was produced.

参考文献/References:

[1]何磊,杨胜强,孙祺,等.Y型通风下采空区瓦斯运移规律及治理研究[J].中国安全生产科学技术,2011,7(2):50-54. HE Lei, YANG Shengqiang, SUN Qi, et al. Study on gas migration rule and control in goaf of Y type ventilation[J]. Journal of Safety Science and Technology, 2011, 7(2): 50-54.
[2]柯为.治理煤矿瓦斯爆炸的微生物技术[J]. 生物工程学报, 2005, 21(3):460-460. KE Wei. Treatment of coal mine gas explosion by microbial technology[J]. Chinese Journal of biotechnology, 2005, 21(3): 460-460.
[3]Chakravorty R.N., Forrester P.I. Application of biotechnology for methane control in coal mines [C]. In: Second US Mine Ventilation Symposium, Reno, Nevada, USA. 23-25, September. Mo usset-Jones P, Balkema AA (eds)Rottesdam, 1985: 699-706.
[4]陈东科,王璐,金龙哲,等.微生物降解煤矿瓦斯的研究[J].煤炭学报,2006.10,31(5):607-609. CHEN Dongke, WANG Lu, JIN Longzhe, et al. Study on degradation of coal mine gas by microbial[J]. Journal of China Coal Society, 2006.10,31(5):607-609.
[5]毛飞.微生物技术治理煤层瓦斯理论及应用研究[D].重庆大学,2013:108-111.
[6]张瑞林,任学清.不同压力及氧环境条件下微生物降解煤层瓦斯实验研究[J].煤矿安全,2014,45(11):1-4. ZHANG Ruilin, REN Xueqing. Experimental study on methane degradation of coal seam under different pressure and oxygen concentration [J]. Safety in Coal Mines,2014,45(11):1-4.
[7]邓湘雯,杨晶晶,陈槐,等.森林土壤氧化(吸收)甲烷研究进展[J].生态环境学报, 2012,21(3):577-583. DENG Xiangwen, YANG Jingjing, CHEN Huai, et al. Progress in the study of oxidation (absorption)of methane in forest soils[J]. Ecology and Environmental Sciences, 2012,21(3):577-583.
[8]佘晨兴,仝川.自然湿地土壤产甲烷菌和甲烷氧化菌多样性的分子检测[J].生态学报,2011,31(14):4126-4135. SHE Chenxing, TONG Chuan. Molecular detection of the diversity of methane producing bacteria and methane oxidizing bacteria in natural wetland soil[J]. Acta Ecologica Sinica, 2011, 31(14): 4126-4135.
[9]Wise MG, McArthur JV.2001. Methylosarcina fibrata gen.nov., s p.nov.and Methylosarcina quisquiliarum sp.nov., novel type I methano-trophs[J]. Int.J.Syst.Evol.Microbiol.,51:611-621.
[10]汤玉平,高俊阳,赵克斌,等.典型油气藏上方甲烷氧化菌群落的分子生物学解析[J].石油实验地质,2014,36(5):605-611. TANG Yuping, GAO Junyang, ZHAO Kebin, et al. Molecular biological analysis of methane oxidizing bacteria community above typical oil and gas reservoirs[J]. Petroleum Geology & Experiment, 2014, 36(5): 605-611.
[11]ArjanPol, Klaas Heijmans, Dario Tedesco, et al. Methanotrophy below pH 1 by a new Verrucomi crobia species[J]. Nature, 2007. 450: 874-881.
[12]Marina V. Omelchenko, Leana V. Vasilyeva, and G .A. Zavarizin. Psychrophilic Methanotroph from Tundra Soil[J]. Current Microbiology, 1993, 27: 255-259.
[13]Hanson R S. Hanson T E.M et hanotrophic Bact eria[J]. Microbiological Reviews.1996, 60(2): 439-471.
[14]Bodrossy L, Holmes E M. Analysis of 16S rRNA and methane monooxygenase gene sequences reveals a novel group of thermotolerant and thermophilic methanetrophs, Methylocaldum gen.nov[J]. Arch. Microbiol. 1997. 168: 493-503.
[15]魏素珍.甲烷氧化菌及其在环境治理中的应用[J].应用生态学报,2012,23(8):2309-2318. WEI Suzhen. Methane oxidizing bacteria and its application in environmental management[J]. Chinese Journal of Applied Ecology, 2012, 23 (8): 2309-2318.
[16]孟召平,田永东,李国富.煤层气开发地质学理论与方法[M].北京:科学出版社,2010:154-159.

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备注/Memo

备注/Memo:
国家自然科学基金项目(51474094)
更新日期/Last Update: 2016-05-05